Journal: Emerging microbes & infections

Article Title: Identification of cepharanthine as an effective inhibitor of African swine fever virus replication.

doi: 10.1080/22221751.2024.2429624

Figure Lengend Snippet: Figure 1. Screening of antiviral compounds against ASFV in the Selleck natural compounds library. (A) Depiction of the screening process of anti-ASFV compounds. A total of 803 compounds were screened in ASFV infected PAMs (1 MOI) using a cell-based Elisa assay. Two replicates were set for each compound at a concentration of 10 μM, and those with high cytotoxicity were excluded in the second round of screening. (B) The screening results of anti-ASFV compounds. Each dot represents a compound, ranging by infection ratio. (C) The 50% cytotoxicity concentrations (CC50) of candidate anti-ASFV compounds. PAMs (2 × 105 cells/well) were seeded in a 96 well plate overnight, treated with the indicated concentration of DMSO or compounds for 24 h, and then treated with CCK-8 agent for an additional 2 h. The OD450 value was measured for cell viability detection. (D) The Half Maximal Inhibitory Concentration (IC50) of candidate anti-ASFV compounds. PAMs were treated with the indicated concentration of DMSO or com pounds for 2 h, followed by a cell-based ELISA assay. Data were calculated through nonlinear regression analysis.

Article Snippet: Screening of antiviral compounds against ASFV in the Selleck natural compounds library. (A) Depiction of the screening process of anti-ASFV compounds.

Techniques: Infection, In-Cell ELISA, Concentration Assay, CCK-8 Assay

Journal: Disease Models & Mechanisms

Article Title: A small-molecule screen identifies that elafibranor links mechanical cues and Irf6-dependent epithelial differentiation

doi: 10.1242/dmm.052649

Figure Lengend Snippet: Carbohydrate metabolism small-molecule screen to identify modulators of irf6 periderm regulation in zebrafish embryos. (A) Schematic of the high-throughput chemical screen in which irf6 maternal-null (m-null) embryos were incubated with each of the compounds in a 384-well format. To visualize enveloping layer (EVL) instability leading to embryo rupture at the onset of epiboly, propidium iodide (PI) was included in the buffer to enable fluorescent readout and quantification using a plate reader. Created in BioRender by Molteni, H. L. (2026). https://BioRender.com/r2ydfea . This figure was sublicensed under CC-BY 4.0 terms. (B) Plot showing average PI fluorescence over early development [4-12 h post-fertilization (hpf)] of wild-type and irf6 m-null embryos exposed to 10 µM elafibranor or DMSO vehicle control. Data are mean±s.e.m. n =48 embryos across three experiments. (C) Heat map of wild-type and irf6 m-null controls (DMSO) and 18 selected compounds that delayed irf6 m-null rupture from plate reader data. Dashed line denotes the time of rupture. All conditions were run in at least three replicates. n =3 embryos. (D) Representative brightfield images of irf6 m-null and wild-type embryos treated with DMSO or 10 µM elafibranor at the following developmental timepoints: sphere, dome, 50% epiboly and bud stage. DMSO-treated irf6 m-null embryos failed to initiate epiboly, rupturing (denoted with an ‘X’) during the sphere stage. Elafibranor delayed rupture time, allowing irf6 m-null embryos to undergo epiboly initiation before rupturing at 50% epiboly. Images taken during mid-rupture are in the black-outlined box to the right of the ‘X’. Wild-type embryos treated with 10 µM elafibranor did not rupture and progressed through epiboly. n =75 embryos across three independent experiments. Scale bar: 200 µm.

Article Snippet: Small molecules were obtained from the Carbohydrate Metabolism Compound Library (Selleck Chem) and stored at −80°C prior to use.

Techniques: High Throughput Screening Assay, Incubation, Fluorescence, Control